SpectraMax iD3/iD5 Multi-Mode Microplate Readers
Product Advantages
- Absorbance
- Fluorescence (top/bottom read)
- Luminescence (top/bottom read)
- Time-Resolved Fluorescence (top/bottom read)
- Fluorescence Polarization (top/bottom read) (id5)
- HTRF (top/bottom read) (id5)
- HTRF(id5)
Product Details
NFC-based personalization and enhanced touchscreen workflows provide walk-up usability
The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.
With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the readers provide an all access pass for breakthrough research.
Personalize workflows
Built-in near-field communication (NFC) functionality can access custom protocols and results with a single tap, saving time better spent on research.
Simplify tasks
The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.
Reduce background noise
The optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) detector that reduces background noise for greater sensitivity and a wide dynamic range.
Features
QuickSync
The QuickSync feature pushes data to any computer within the same network and delivers data in an Excel-compatible format.
Flexible temperature control
Simple-to-use temperature control adjusts the experiment's conditions from ambient up to 66°C.
Automatic filter identification
Using NFC tags, the SpectraMax iD5 reader automatically detects the wavelength properties of each filter in addition to the filter slide configuration, eliminating confusion and the need for manual information entry.
Enhanced security
The SoftMax® Touch Software secures user accounts with PIN- or NFC-protection with a lock screen option. Data is stored on the reader’s hard drive ensuring security even after transfer to a computer.
Hybrid optical system
The SpectraMax iD5 reader provides the flexibility to set up experiments using filters, or a monochromator in 1 nm increments, or a hybrid mix of monochromator and filter-based wavelength selection.
Flash assays captured with ease
The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 µL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.
Please visit Molecular Devices' official website for more information.
Specifications
Brand: Molecular Devices
| Model | SpectraMax iD3 Multi-Mode Microplate Reader | SpectraMax iD5 Multi-Mode Microplate Reader |
| Read modes | Absorbance Fluorescence (top/bottom read) Luminescence (top read) |
Absorbance Fluorescence (top/bottom read) Luminescence (top/bottom read) Time-Resolved Fluorescence (top/bottom read) Fluorescence Polarization (top/bottom read) HTRF (top/bottom read) HTRF |
| Wavelength ranges | Abs: 230 - 1000 nm FL Ex: 250 - 830 nm FL Em: 270 - 850 nm Lumi: 300 - 850 nm |
Abs: 230 - 1000 nm FL Ex: 250 - 830 nm FL Em: 270 - 850 nm Lumi: 300 - 850 nm TRF Ex: 350 nm TRF Em: 450 - 750 nm FP: 300 - 750 nm |
| Wavelength selection | Monochromator tunable in 1-nm increments | Hybrid filters and monochromator tunable in 1 nm increments |
| Absorbance photometric accuracy/linearity | < ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV | < ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV |
| Absorbance photometric precision/repeatability | < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV | < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV |
| Sensitivity top read optimized | FL: 1 pM fluoresein 96 well, 1 pM fluorescein 384 well Lumi: 2 pM ATP 96 well, 4 pM |
FL: Mono: 1 pM fluorescein 96 well, 1 pM fluorescein 384 well; Filters: 0.3 pM fluoresein 96 well, 0.5 pM fluorescein 384 well Lumi: 2 pM ATP 96 well, 4 pM ATP 384 well TRF (filters with enhanced TRF module): 10 fM Europium (2 amol/well) 96 well, 10 fM Europium (1 amol/well) 384 well FP: Mono: 2 mP @ 10nM 96 well, 2 mP @ 10nM 384 well; Filters: 1 mP @ 1 nM 96 well, 2 mP @ 1 nM 384 well |
| Light source(s) | High power xenon flash lamp | High power xenon flash lamp |
| Detector(s) | Silicon photodiode Ultra-cooled photomultiplier tube |
Silicon photodiode Ultra-cooled photomultiplier tube |
| Read types (endpoint, kinetic, etc) | Endpoint Kinetic Spectrum scan Well scan |
Endpoint Kinetic Spectrum scan Well scan |
| Read times | Abs: 28 seconds FL: 21 seconds Lumi: 21 seconds |
Abs: 28 seconds FL: 21 seconds Lumi: 21 seconds TRF: 25 seconds FP: 35 seconds |
| Plate shaking | Linear Orbital Double orbital |
Linear Orbital Double orbital |
| Injectors | ✓ | ✓ |
| Western blot | - | ✓ |
| Stand-alone touchscreen operation | ✓ | ✓ |
| Robotics / automation | ✓ | ✓ |
| Plate type(s) | 6-384 well plates | 6-384 well plates |
| Temperature control | ✓ Ambient + 5°C to 66°C |
✓ Ambient + 5°C to 66°C |
| Dimensions | H 40.1 cm x W 53.2 cm x D 59.8 cm | H 40.1 cm x W 53.2 cm x D 59.8 cm |
| Validation tools | ✓ Absorbance validation plate Fluorescence validation plate Luminescence validation plate SoftMax® Pro GxP Software |
✓ Absorbance validation plate Fluorescence validation plate Luminescence validation plate Multi-mode validation plate SoftMax® Pro GxP Software |