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ImageXpress® HCS.ai – High-Content Imaging System
Deeper Insights. Smarter Science.
Empowering Discovery with AI and Advanced Imaging
The ImageXpress® HCS.ai system combines cutting-edge hardware with AI-driven analysis to deliver high-resolution, multi-dimensional imaging for 2D and 3D applications. Seamlessly integrated with MetaXpress® Acquire for image acquisition and IN Carta® for AI-powered analysis, this platform accelerates your research with flexibility, scalability, and precision.
Product Features
- High-performance Camera
Advanced camera module for faster image capture and superior resolution—accelerating high-throughput workflows. - AgileOptix™ Spinning Disk Technology
Switch between high speed and high resolution modes for optimal imaging under any experimental condition. Offers up to 12 magnification options for maximum flexibility with magnification changer. - Multiplex Imaging
Supports brightfield, widefield, and confocal fluorescence for 2D, 3D, and live-cell imaging. - Water Immersion Option
Boosts signal intensity up to 4x, reduces exposure time, and minimizes phototoxicity—ideal for long-term live-cell studies. - High-Power Laser Illumination
Up to 7 lasers and 8 detection channels for complex multiplexed experiments with high accuracy. - AI-Powered Analysis
Integrated IN Carta® software uses machine learning to automatically identify and classify cellular features—reducing manual effort and improving consistency. - MetaXpress® Acquire Software
Intuitive interface for rapid setup of imaging and analysis workflows—lowering the learning curve and increasing efficiency. - Modular Design
Field Upgradable lenses, light sources, and automation modules on-site to meet evolving research needs. - Automation Ready
Supports walkaway automation—process up to 40 plates (96-well) in just 2 hours, dramatically increasing throughput and reducing labor.
Brand: Molecular Devices
Model: ImageXpress HCS.ai
| Focus |
• High-speed hardware autofocus with multi-surface detection • Integrated image autofocus option |
| Camera |
• >5 mega pixel sCMOS sensor with large sensor size (224mm²) • >3 log dynamic range with 16bit detector, 95% peak quantum efficiency |
| Stage | Linear encoded voice coil driven X, Y, and Z stages with better than 25 nm resolution |
| Filters |
• 4-position software selectable excitation and dichroic filter wheel • 10-position software selectable emission filter wheel |
| Transmitted light | High-contrast imaging where unstained cells are easily viewed or separated from background |
| Objectives |
• 6-position automated objective changer • High NA air objectives: 2X–60X (up to 0.85 NA) • Extra long working distance objectives 20X–60X (up to 0.7 NA) • Water immersion (20x, 40x, 60x) |
| Sample compatibility | Microplates (1–1536 wells), round or flat bottom, low to high profile, slide holder |
| Magnification changer (optional) |
• 1X (standard) and 1.5X modifies objective capability: to provide 2X–90X magnification • Automated switching with software control |
| Water immersion (optional) |
• 20X, 40X, 60X objectives available (up to 1.2 NA) • Increase signal up to 4 times for brighter intensity at lower exposure times • Increase in penetration depths dependent on sample • Improve z-resolution and decrease optical aberrations • Auto water replenishment enables screening or imaging across a plate |
| Environmental Control (optional) |
• Multi-day, live cell time-lapse imaging • Controls for appropriate atmospheric conditions • Temperature: heating up to 40 °C • Integrated gas mixer: CO2 (0–15%), O2 (1–21%) • Controls humidity and minimizes evaporation (0.5 μL/well/hour for 96- or 384-well formats) • Robot-friendly loading design |
| Autofocus | Hardware + Image autofocus |
| Plates supported | Up to 1536-well plates |
| Temperature control | Yes |
| 96-Well Plate Scan Time, 2 Colors, 1 Site/Well | 1.5 minutes |
| Maximum Illumination Channels | 7 |
| Robotics/Automation-compatible | Yes |