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SpectraMax i3x Multi-Mode Microplate Reader
Unlimited breadth of user-installable application modules expands research capabilities
The SpectraMax® i3x Multi-Mode Microplate Reader can perform a wide range of essential cell-based and biochemical assays using the included absorbance, fluorescence, and luminescence detection modes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, this unique reader enables users to add optional detection modules at any time to expand the reader’s capabilities and explore cellular pathways and protein expression on one microplate reader. This includes AlphaScreen/AlphaLisa, time-resolved fluorescence, TR-FRET/HTRF, fluorescence polarization, dual injectors, and western blot detection. Transmitted light and fluorescence channels offer the option to image stain-free or use cellular dyes to get a better view of cytotoxicity, cell proliferation, and phenotypic changes.
Product Features
User-configurable applications
User-installable detection modules expand the reader’s detection capabilities to include AlphaScreen, time-resolved fluorescence, TR-FRET (including HTRF), fast kinetics with injectors, western blot detection, and more.
No-stain cell analysis
The StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell analysis workflow.
Multi-channel functionality
Two fluorescence detection channels on the MiniMax 300 cytometer enable the analysis of cell viability or cell toxicity assays, including viability assays and transfection efficiency.
Cellular pathway analysis
Cell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein, can be captured on one reader.
Spectral Optimization Wizard
This built-in technology enables users to optimize their assay in a single-step by quickly identifying the best excitation and emission wavelengths.
Auto Intensity Adjustment
Our patented technology acquires data over a wide dynamic range without the need to re-read the plate due to saturation effects. PMT gain adjustment is bypassed with the use of high-power LEDs as part of the Spectral Fusion Illumination.
Brand: Molecular Devices
Model: SpectraMax i3x
| Read modes |
Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read) Fluorescence Polarization (top read) HTRF (top read) AlphaScreen (top read) |
| Wavelength ranges |
Abs: 230 - 1000 nm FL Ex: 250 - 830 nm FL Em: 270 - 850 nm Lumi: 300 - 850 nm |
| Wavelength selection | Cartridge filters and monochromator tunable in 1 nm increments |
| Absorbance photometric accuracy/linearity | < ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 2 OD UV |
| Absorbance photometric precision/repeatability | < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 2 OD UV |
| Sensitivity top read optimized | FL: Mono: 0.5 pM fluorescein 96 well, 1 pM fluorescein 384 well; Cartridge: 0.1 fmol/well in 75 μL fluorescein 384 well, 0.03 fmol/well in 8 μL fluorescein 1536 well Lumi: Mono: 3 pM ATP-Glow 96 well, 6 pM ATP-Glow 384 well; Cartridge: 20 amol ATP-flash TRF cartridge: 3 amol/well in 100 μL europium (0.03 pM) 384 well, 1 amol/well in 8 μL europium (0.125 pM) 1535 well FP cartridge: 3 mP (75 μl) fluorescein 384 well, 6 mP (8μl) fluorescein 1536 well AlphaScreen cartridge: <100 amol/well 384 well |
| Light source(s) |
High power xenon flash lamp LEDs in visible range |
| Detector(s) |
Silicon photodiode Cooled photomultiplier tube |
| Read types (endpoint, kinetic, etc) |
Endpoint Kinetic Spectrum scan Well scan |
| Read times |
Abs: 29 seconds FL: 22 seconds Lumi: 21 seconds TRF: 21 seconds FP: 38 seconds AS: 22 seconds |
| Plate shaking |
Linear Orbital |
| Injectors | ✓ |
| Imaging | ✓ |
| Western Blot | ✓ |
| Robotics / automation | ✓ |
| Plate type(s) | 6-1536 well plates |
| Temperature control |
✓ Ambient + 4°C to 45°C |
| Dimensions | H 32.5 cm x W 39.2 cm x D 60.55 cm |
| Validation tools |
Absorbance validation plate Fluorescence validation plate Luminescence validation plate Multi-mode validation plate SoftMax® Pro GxP Software |
| Absorbance, Fluorescence and Luminescence | ✓ |
| Time-Resolved Fluorescence (TRF) | ✓ |
| Fluorescence Polarization (FP) | ✓ |
| Htrf | ✓ |
| Alpha Screen | ✓ |
| Cuvette Port | ✓ |
| Cell Imaging | ✓ |
| Plates | 6-1536 well plates |
| Autmation Ready | |
| GxP Compliance | ✓ |
Technical Specifications for SpectraMax MiniMax 300 Imaging Cytometer
| Light source | Proprietary solid state illumination, White, 460/20 nm and 625/20 nm excitation |
| Detector | 1.25 megapixel, 12-bit high sensitivity CCD camera |
| Emission | Brightfield; Green 541/108 nm; Red 713/123 nm |
| Objective | Single 4X objective |
| Autofocus | Proprietary laser scanning autofocus |
| Resolution | 1.9 μm x 1.9 μm pixel size |
| Data acquisition and analysis software | SoftMax® Pro Software MiniMax Imaging Edition |
| Imaging speed* (1 color - 96 wells) |
Acquisition 3:40 Acquisition + Analysis 6:30 |
| Specimen carriers |
ANSI/SBS-conformant microplates, 96 and 384 wells |
| Dimensions (cm) |
39.2 cm W x 19.5 cm H x 60.6 cm D (SpectraMax® MiniMax™ 300 Imaging Cytometer) 39.2 W x 44.0 H x 60.6 L (with base system) |