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SpectraMax iD3/iD5 Multi-Mode Microplate Readers

SpectraMax iD3/iD5 Multi-Mode Microplate Readers

Product Advantages

  • Absorbance
  • Fluorescence (top/bottom read)
  • Luminescence (top/bottom read)
  • Time-Resolved Fluorescence (top/bottom read)
  • Fluorescence Polarization (top/bottom read) (id5)
  • HTRF (top/bottom read) (id5)
  • HTRF(id5)

 

 

Product Details

NFC-based personalization and enhanced touchscreen workflows provide walk-up usability

 

The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.

 

With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the readers provide an all access pass for breakthrough research.

 

Personalize workflows

Built-in near-field communication (NFC) functionality can access custom protocols and results with a single tap, saving time better spent on research.

 

Simplify tasks

The intuitive touchscreen software simplifies tasks with Quick Read icons that save time when starting new experiments and a personalized library that stores frequently used protocols.

 

Reduce background noise

The optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) detector that reduces background noise for greater sensitivity and a wide dynamic range.

 

Features

  • QuickSync

    The QuickSync feature pushes data to any computer within the same network and delivers data in an Excel-compatible format.

     

  • Flexible temperature control

    Simple-to-use temperature control adjusts the experiment's conditions from ambient up to 66°C.

     

  • Automatic filter identification

    Using NFC tags, the SpectraMax iD5 reader automatically detects the wavelength properties of each filter in addition to the filter slide configuration, eliminating confusion and the need for manual information entry.

     

  • Enhanced security

    The SoftMax® Touch Software secures user accounts with PIN- or NFC-protection with a lock screen option. Data is stored on the reader’s hard drive ensuring security even after transfer to a computer.

     

  • Hybrid optical system

    The SpectraMax iD5 reader provides the flexibility to set up experiments using filters, or a monochromator in 1 nm increments, or a hybrid mix of monochromator and filter-based wavelength selection.

     

  • Flash assays captured with ease

    The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 µL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.

 

Please visit Molecular Devices' official website for more information.

Brand: Molecular Devices

 

Model SpectraMax iD3 Multi-Mode Microplate Reader SpectraMax iD5 Multi-Mode Microplate Reader
Read modes Absorbance
Fluorescence (top/bottom read)
Luminescence (top read)
Absorbance
Fluorescence (top/bottom read)
Luminescence (top/bottom read)
Time-Resolved Fluorescence (top/bottom read)
Fluorescence Polarization (top/bottom read)
HTRF (top/bottom read)
HTRF
Wavelength ranges Abs: 230 - 1000 nm
FL Ex: 250 - 830 nm
FL Em: 270 - 850 nm
Lumi: 300 - 850 nm
Abs: 230 - 1000 nm
FL Ex: 250 - 830 nm
FL Em: 270 - 850 nm
Lumi: 300 - 850 nm
TRF Ex: 350 nm
TRF Em: 450 - 750 nm
FP: 300 - 750 nm
Wavelength selection Monochromator tunable in 1-nm increments Hybrid filters and monochromator tunable in 1 nm increments
Absorbance photometric accuracy/linearity < ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV < ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
Absorbance photometric precision/repeatability < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
Sensitivity top read optimized FL: 1 pM fluoresein 96 well, 1 pM fluorescein 384 well
Lumi: 2 pM ATP 96 well, 4 pM
FL: Mono: 1 pM fluorescein 96 well, 1 pM fluorescein 384 well; Filters: 0.3 pM fluoresein 96 well, 0.5 pM fluorescein 384 well
Lumi: 2 pM ATP 96 well, 4 pM ATP 384 well TRF (filters with enhanced TRF module): 10 fM Europium (2 amol/well) 96 well, 10 fM Europium (1 amol/well) 384 well
FP: Mono: 2 mP @ 10nM 96 well, 2 mP @ 10nM 384 well; Filters: 1 mP @ 1 nM 96 well, 2 mP @ 1 nM 384 well
Light source(s) High power xenon flash lamp High power xenon flash lamp
Detector(s) Silicon photodiode
Ultra-cooled photomultiplier tube
Silicon photodiode
Ultra-cooled photomultiplier tube
Read types (endpoint, kinetic, etc) Endpoint
Kinetic
Spectrum scan
Well scan
Endpoint
Kinetic
Spectrum scan
Well scan
Read times Abs: 28 seconds
FL: 21 seconds
Lumi: 21 seconds
Abs: 28 seconds
FL: 21 seconds
Lumi: 21 seconds
TRF: 25 seconds
FP: 35 seconds
Plate shaking Linear
Orbital
Double orbital
Linear
Orbital
Double orbital
Injectors
Western blot -
Stand-alone touchscreen operation
Robotics / automation
Plate type(s) 6-384 well plates 6-384 well plates
Temperature control
Ambient + 5°C to 66°C

Ambient + 5°C to 66°C
Dimensions H 40.1 cm x W 53.2 cm x D 59.8 cm H 40.1 cm x W 53.2 cm x D 59.8 cm
Validation tools
Absorbance validation plate
Fluorescence validation plate
Luminescence validation plate
SoftMax® Pro GxP Software

Absorbance validation plate
Fluorescence validation plate
Luminescence validation plate
Multi-mode validation plate
SoftMax® Pro GxP Software
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