SpectraMax® iD3s – Multi-Mode Microplate Reader
Compact. Versatile. Reliable.
Your Everyday Solution for Life Science and Drug Discovery
The SpectraMax® iD3s combines three detection modes in one streamlined system, delivering flexibility and precision for a wide range of assays. Designed for efficiency and ease of use, it supports everything from routine measurements to advanced live-cell experiments.
Why Choose SpectraMax® iD3s?
- Three-in-One Detection
Absorbance, fluorescence, and luminescence in a single platform—covering diverse experimental needs. - High-Precision Monochromator
1 nm wavelength resolution for accurate and flexible spectral selection. - Temperature Control
Maintains stable conditions from ambient +5°C to 66°C for consistent results. - Live-Cell Ready
Optional SpectraMax® Aer Gas Controller and advanced shaking/orbital mixing for optimal culture conditions. - SmartInject® Dual Injectors
Enables rapid, uniform mixing—ideal for kinetic and dynamic assays. - SoftMax® Pro Software
Includes 200+ preconfigured protocols for simplified setup and analysis. - Optional Accessories
- SpectraDrop™ for ultra-low-volume samples
- StakMax® Microplate Stacker for high-throughput workflows (20+ plates)
- Regulatory Compliance
Supports IQ/OQ and GxP validation for regulated environments.
Brand: Molecular Devices
Model: SpectraMax iD3s
| Feature | Specification |
| Read modes |
Absorbance Fluorescence (top/bottom read) Luminescence (top read) |
| Wavelength ranges |
Abs: 230 - 1000 nm FL Ex: 250 - 830 nm FL Em: 270 - 850 nm Lumi: 300 - 850 nm |
| Wavelength selection | Monochromator tunable in 1-nm increments |
| Absorbance photometric accuracy/linearity | < ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV |
| Absorbance photometric precision/repeatability | < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV |
| Sensitivity top read optimized |
FL: 1 pM fluoresein 96 well, 1 pM fluorescein 384 well Lumi: 2 pM ATP 96 well, 4 pM |
| Light source(s) | High power xenon flash lamp |
| Detector(s) |
Silicon photodiode Ultra-cooled photomultiplier tube |
| Read types (endpoint, kinetic, etc) |
Endpoint Kinetic Spectrum scan Well scan |
| Read times |
Abs: 28 seconds FL: 21 seconds Lumi: 21 seconds |
| Plate shaking |
Linear Orbital Double orbital |
| Injectors | Yes |
| Western blot | No |
| Robotics / automation | Yes |
| Plate type(s) | 6-384 well plates |
| Temperature control | Yes Ambient + 5°C to 66°C |
| Dimensions | H 40.1 cm x W 53.2 cm x D 59.8 cm |
| Validation tools |
Yes Fluorescence validation plate Luminescence validation plate SoftMax® Pro GxP Software |
| SpectraMax aer gas mixer (optional) |
O2 control range: 1-21% in 0.1% increments CO2 control range: 0.1-15% in 0.1% increments O2 accuracy: ±1.0% (37°C, 1% O2) CO2 accuracy: ±1.0% (37°C, 5% CO2) |




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