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SpectraMax® iD5e – Advanced Multi-Mode Microplate Reader
High Sensitivity. Expanded Capabilities. Maximum Flexibility.
Designed for Advanced Life Science and Drug Discovery Applications
The SpectraMax® iD5e delivers five detection modes in one powerful platform, offering unmatched versatility for complex assays. With enhanced sensitivity, hybrid optics, and advanced environmental controls, it supports cutting-edge applications while maintaining ease of use and regulatory compliance.
Why Choose SpectraMax® iD5e?
- Five-in-One Detection
Absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and fluorescence polarization (FP). - Expanded Application Support
Compatible with TR-FRET, HTRF®, BRET, and Western blot detection. - Hybrid Optical System
Combines monochromators and filters with ultra-cooled PMT for superior sensitivity and dynamic range. - Live-Cell Assay Support
Optional CO₂/O₂ gas mixer and advanced shaking maintain ideal conditions for cell-based and microbial assays. - SmartInject® Dual Injectors
Enables rapid, uniform mixing for kinetic and flash assays. - Touchscreen Interface
Intuitive, personalized touchscreen with Quick Read icons and protocol library for faster workflows. - Automatic Filter Identification
Built-in NFC technology simplifies setup and ensures accuracy. - Network Connectivity
QuickSync™ technology for seamless data transfer and integration. - Temperature Control
From ambient +5°C to 66°C for temperature-sensitive assays. - Auto Z-Height Optimization
Maximizes sensitivity and reproducibility, even with low-volume samples. - Optional Accessories
- SpectraDrop™ for ultra-low-volume samples
- StakMax® Microplate Stacker for high-throughput workflows (20+ plates)
- Regulatory Compliance
Supports IQ/OQ and GxP validation for regulated environments.
Brand: Molecular Devices
Model: SpectraMax iD5e
| Feature | Specification |
| Read modes |
Absorbance Fluorescence (top/bottom read) Luminescence (top/bottom read) Time-Resolved Fluorescence (top/bottom read) Fluorescence Polarization (top/bottom read) HTRF (top/bottom read) HTRF |
| Wavelength ranges |
Abs: 230 - 1000 nm FL Ex: 250 - 830 nm FL Em: 270 - 850 nm Lumi: 300 - 850 nm TRF Ex: 350 nm TRF Em: 450 - 750 nm FP: 300 - 750 nm |
| Wavelength selection | Hybrid filters and monochromator tunable in 1 nm increments |
| Absorbance photometric accuracy/linearity | < ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV |
| Absorbance photometric precision/repeatability | < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV |
| Sensitivity top read optimized |
FL: Mono: 1 pM fluorescein 96 well, 1 pM fluorescein 384 well; Filters: 0.3 pM fluoresein 96 well, 0.5 pM fluorescein 384 well
Lumi: 2 pM ATP 96 well, 4 pM ATP 384 well TRF (filters with enhanced TRF module): 10 fM Europium (2 amol/well) 96 well, 10 fM Europium (1 amol/well) 384 well
FP: Mono: 2 mP @ 10nM 96 well, 2 mP @ 10nM 384 well; Filters: 1 mP @ 1 nM 96 well, 2 mP @ 1 nM 384 well |
| Light source(s) | High power xenon flash lamp |
| Detector(s) |
Silicon photodiode Ultra-cooled photomultiplier tube |
| Read types (endpoint, kinetic, etc) |
Endpoint Kinetic Spectrum scan Well scan |
| Read times |
Abs: 28 seconds FL: 21 seconds Lumi: 21 seconds TRF: 25 seconds FP: 35 seconds |
| Plate shaking | Linear Orbital Double orbital |
| Injectors | Yes |
| Western blot | Yes |
| Robotics / automation | Yes |
| Plate type(s) | 6-384 well plates |
| Temperature control | Yes Ambient + 5°C to 66°C |
| Dimensions | H 40.1 cm x W 53.2 cm x D 59.8 cm |
| Validation tools |
Yes Fluorescence validation plate Luminescence validation plate Multi-mode validation plate SoftMax® Pro GxP Software |
| SpectraMax aer gas mixer (optional) |
O2 control range: 1-21% in 0.1% increments CO2 control range: 0.1-15% in 0.1% increments O2 accuracy: ±1.0% (37°C, 1% O2) CO2 accuracy: ±1.0% (37°C, 5% CO2) |